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Cephalexin is a kind of β -lactam antibiotics whose annual production is about 30 thousand tons with sales of USD 15 billion [1]
The hydrolysis of cephalexin, cephalothin and cephaloridine in strongly acid medium (10--25% sulfuric acid) at high temperature (approximately 100 degrees C) was studied
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Yields of cephalexin were 21% (in the aqueous system) and 60% (in the ATPS), respectively
By carrying out the reaction in 18O Cephalexin was synthesized in aqueous two-phase systems (ATPSs) by using penicillin G acylase (PGA The efficient mutant βF24A was selected, and the (P s /P h) ini (ratio between the initial rate of synthesis and hydrolysis of the activated acyl donor) dramatically increased from 1
Historically, many cellulase kinetic models, established for predicting the initial reaction rate of cellulose hydrolysis, have in general worked satisfactorily [4], [5]
Substrates and products of hydrolysis of penicillin G and synthesis of cephalexin were identified and analyzed by HPLC using a Jasco delivery system PU-2089plus with a Jasco UV 2075 UV–Vis detector and a LC-NetII/ADC Jasco HPLC/PC integrator
After the process the solutions were analyzed by high-performance liquid chromatography (HPLC)/ESI-MS
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This results in a temporary high-product concentration that is subsequently consumed by the enzyme
In neutral and weak alkaline The kinetics of cephalexin synthesis and hydrolysis of the activated acyl-donor precursor PGME were characterized under a broad range of substrate concentrations
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Cephalexin (CEX) was synthesized with 7-amino-3-deacetoxycephalosporanic acid and D(–)
Study on Thermodynamics and Kinetics of Cephalexin Enzymatic Hydrolysis and Its Process Development to Prepare 7-ADCA
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The maximal enzyme activity during L-Ala-pNA and cephalexin hydrolysis is manifested at pH 6
The molecular mass of the enzyme is 270-280 kDa