Objectives: To establish a human model of Aldara-induced psoriasis-like skin inflammation in patients with psoriasis
Hence, recent
Imiquimod (IMQ) is a small nucleotide-like molecule commonly used to induce psoriasis-like dermatitis in animal models (Swindell et al
BALB/c mice received the drug (5% Aldara) at a dose
The modified IMQ-model reproduces psoriatic skin alterations highlighting considerably
The Aldara ® induced psoriasis model is widely used because of its rapid development, low cost and simple read out
In mice, the topical application of imiquimod (IMQ), a ligand of Toll-like receptors (TLR) 7 and 8, has been reported to induce psoriasis-like dermatitis [ 3 ]
It started on Jan
In the Aldara-induced psoriasis model, the cells accumulating in IL-12-deficient tissue are predominantly Vγ6 + type 17 cells
February 20, 2024 2:26pm
The imiquimod-induced psoriasis model appears to be useful for the investigation of itch and its sensitization in psoriasis
Recent studies have shown that Aldara cream, used to treat benign skin abnormalities, triggers psoriasis-like disease in humans and mice and have implicated Th17 cells in disease initiation
We chose nonlesional psoriatic skin of patients with psoriasis rather than normal skin of patients free of skin disease to be sure that the genetic predisposition for psoriasis was present in the Many autoimmune disorders such as psoriasis lead to the alteration of skin components which generally manifests as unwanted topical symptoms
Article Snippet: Psoriasis-like skin inflammation was induced by applying a daily dose of 45 mg of 5% imiquimod cream (Aldara™ 5%; Meda AB, Solna, Sweden) topically onto the shaved back of the mouse for 7 consecutive days
The IMQ-induced dermatitis model phenocopies important features of psoriasis, including scaling, erythema, and acanthosis ( van der Fits et al
Methods We first set up imiquimod-induced dermatitis in mice that closely resembles human psoriasis
Imiqui-mod is a ligand for the Toll-Like Receptors (TLR) 7 and 8
Attenuated inflammation was associated with reduced dermal macrophage influx and production of
The study of psoriasis has been hindered by the lack of a mouse phenotype that fully recapitulates the complex features of lesions in psoriasis patients [1, 2]
5 mg Aldara is smeared onto the shaved dorsal skin of mice for 5 or 6 days to induce scaly skin lesions resembling plaque type psoriasis
Some models translate better to human disease, and many have provided unique insight into psoriasis disease pathogenesis
Methods: We used single-cell RNA sequencing (10× Genomics) as an unbiased analytical strategy to investigate the heterogeneity of skin immune cells in imiquimod-induced psoriasis mice systematically
Advantages of this model include rapid development of the skin response and cost-effectiveness
The application of Aldara ® cream containing 5% imiquimod stimulates Toll-like receptor 7/8 on plasmacytoid dendritic cells, thereby producing a potent immunomodulatory effect