I inoculated 20 mL of Rosetta pre-culture into 1L LB broth supplemented with 1 mL of Kanamycin and 1 mL of Chloramphenicol
They are usually on a The transformed cells were plated in LB agar containing 50 μg/μL of kanamycin and additional 25 μg/μL of chloramphenicol in the cases of
E
Rosetta™ and Rosetta 2 host strains are BL21 derivatives designed to enhance the expression of eukaryotic proteins that contain codons rarely used in E
Incubate the plates inverted at 37ºC O/N
Rosetta host strains are BL21 derivatives designed to enhance the expression of eukaryotic proteins that contain codons rarely
Pseudomonas putida KT2440 is a chloramphenicol-resistant bacterium that is able to grow in the presence of this antibiotic at a concentration of up to 25 μg/ml
Chloramphenicol is an antibiotic and is in the class of antimicrobials that inhibits protein synthesis